This protocol provides a detailed procedure for determining cell cycle status of tissue culture cells through double staining of Ki-67 and PI using flow cytometry.

This guide integrates fundamental flow cytometry principles, step-by-step workflows, and advanced strategies to empower researchers in designing robust gating protocols.

Oct 8, 2024 · Single cell gating is the place to draw tight gates. It’s important, here, to exclude events which are extra wide (the Width parameter) and extra tall (the Height parameter) which identify cells …

Flow cytometry with propidium iodide (PI) staining is a widely used technique for analyzing DNA content and assessing cell cycle distribution. This protocol outlines a reliable method for staining fixed or …

Nov 5, 2024 · Discover essential strategies for effective flow cytometry gating. This guide explores single-cell gating, dead cell exclusion, doublet discrimination, and population-specific gating …

4 days ago · Flow cytometry is a laboratory technique that measures the physical and chemical characteristics of cells as they flow in a fluid suspension past a laser beam. The process generates a …

Cell cycle analysis by flow cytometry determines the distribution of cells across the various stages of proliferation. This method measures the amount of DNA in individual cells, providing a snapshot of a …

This blog post will take you through the various gating strategies for effective flow cytometry analysis.

A step-by-step guide to flow cytometry gating for easy analysis and clean, publication-ready data. Written by Michelle Belmont, Scientist, MS at Proteintech Group.